Thesis - An innovative combination of experimental and modeling approcaches to assess the role of amibes in the spread of antibiotic resistance in soil (M/F)

Descrizione

Thesis - An innovative combination of experimental and modeling approcaches to assess the role of amibes in the spread of antibiotic resistance in soil (M/F) Sintesi in italiano (traduzione automatica): L'Institut des Géosciences de l'Environnement (IGE) di Grenoble, Francia, cerca un ricercatore per una tesi di dottorato focalizzata sul ruolo delle amebe nella diffusione della resistenza agli antibiotici nel suolo. Il candidato dovrà utilizzare un approccio interdisciplinare 'One Health' per studiare come le proprietà del suolo influenzano la sopravvivenza e la mobilità di amebe e batteri resistenti agli antibiotici. Le mansioni principali includono la progettazione e l'esecuzione di esperimenti, l'uso di modelli di simulazione e l'analisi dei dati. È richiesta una laurea in scienze ambientali o un campo correlato. La posizione è situata nel campus universitario di Grenoble, in un ambiente di ricerca dinamico e collaborativo. The Institut des Géosciences de l'Environnement (IGE, http://www.ige-grenoble.fr ) is a French public research establishment under the supervision of CNRS Terre & Univers, IRD, Université Grenoble Alpes (UGA), Grenoble-INP and INRAE. It employs some 330 people, including 190 permanent members (researchers, associate and full professors, research engineers), and around 140 PhD students, post-doctoral researchers and staff on fixed-term contracts. The Institute also welcomes several dozen interns and visiting scientists every year. The Institute is located on three sites on the Grenoble University Campus (Molière, OSUG-B and Maison Climat Planète). The IGE is one of the main laboratories of the Observatoire des Sciences de l'Univers de Grenoble (OSUG), a federative structure of the Institut National des Sciences de l'Univers (INSU) of the Centre National de la Recherche Scientifique (CNRS). Antibiotic resistance (ABR) is a global threat affecting human, animal, and environmental health. Limiting its environmental reservoirs requires a better understanding of its pathways of dissemination. While wastewater and surface water have been well studied, the role of soil remains largely unexplored. Soils are, however, major reservoirs of antibiotic-resistant bacteria (ARBs) carrying antibiotic resistance genes (ARGs). In this terrestrial environment, protists (micro-eukaryotes), particularly amoebae, play an important role in regulating bacterial populations through their predatory activity. However, certain bacteria—including opportunistic pathogens such as ARBs—resist amoebic lysis after phagocytosis and thus persist within the amoebic microbiota. Amoebae could thus play a key role as vectors of antibiotic-resistant bacteria (genes) (ARBs/ARGs). Through an integrated interdisciplinary “One Health” approach, this thesis proposes to study the influence of soil properties on the survival and mobility of amoebae, ARBs, and ARGs (molecular labeling). An innovative combination of experiments and modeling (reactors, soil cores, X-ray tomography, hydrobiogeochemical model) will enable the quantification of their transport in the soil and the prediction of the risk of AMR dissemination. Annuncio in inglese. Fonte: Euraxess (Commissione europea).