2 PhD positions in the Horizon Europe MSCA Doctoral Network SPACE-MEL

Descrizione

2 PhD positions in the Horizon Europe MSCA Doctoral Network SPACE-MEL Sintesi in italiano (traduzione automatica): L'Erasmus University Medical Centre di Rotterdam offre due posizioni di dottorato nel contesto della rete dottorale MSCA Horizon Europe SPACE-MEL. Il progetto si concentra sullo sviluppo di un flusso di lavoro 3D per il profilo multimodale di modelli di melanoma e campioni di tessuto. I candidati lavoreranno su metodologie innovative per la sequenza di cellule tumorali e immunitarie, integrando tecniche di microscopia e sequenziamento. È richiesta una laurea in biologia, biotecnologie o un campo correlato. I candidati riceveranno formazione pratica e opportunità di collaborazione con esperti del settore, con l'obiettivo di stabilire metodologie avanzate e mappe multimodali delle cellule tumorali in modelli di melanoma 3D. Host institution: Erasmus University Medical Centre Rotterdam (EMC-a), Department of Molecular Genetics , Rotterdam, Netherlands Supervisor: Dr. Miao-Ping Chien Co-supervisors: Prof. Thierry Voet, Katholieke Universiteit Leuven, Department of Human Genetics, Belgium (Academic); Dr. Domenico Bellomo, SkylineDx B.V., Netherlands (Industry) Project description: DC2 will develop a 3D spatial FUN-G&T-seq and/or scNMT-seq workflow for multimodal profiling of melanoma models and tissue samples. The project will translate the protocol developed by DC3 into 3D melanoma organoid and immune cell co-cultures, fresh 200-400 μm tissue sections or explant samples. It pursues two integrated objectives: i) establish a methodology for 3D spatial FUN-G&T-seq and/or spatial scNMT-seq; ii) apply this workflow to profile tumour and immune cells in melanoma organoid co-cultures and live tissue slices. At Erasmus MC, the candidate will work to combine spatial FUNseq with single-cell genome and transcriptome sequencing and/or single-cell nucleosome, methylation and transcriptome sequencing. The workflow will be adapted to 3D light-sheet microscopy and thick melanoma samples, addressing challenges linked to image quality, cell selection, tissue depth and multimodal data integration. During a secondment with Dr. Chien’s group, the candidate will receive training in spatial FUNseq and image-guided single-cell isolation. A second secondment at SkylineDx will explore the possible translation of emerging signatures towards a clinically oriented assay. Expected outcomes include an established 3D spatial FUN-G&T-seq and/or scNMT-seq methodology and multimodal maps of tumour and immune cell states in 3D melanoma models. Annuncio in inglese. Fonte: Euraxess (Commissione europea).