Researcher in synthetic biology and biosynthetic gene cluster expression

Descrizione

Researcher in synthetic biology and biosynthetic gene cluster expression Sintesi in italiano (traduzione automatica): L'Università NTNU, con sede a Trondheim, cerca un ricercatore a tempo pieno per un progetto triennale nel Dipartimento di Biotecnologia e Scienza degli Alimenti. Il ruolo è legato al progetto AIMARIA, che si occupa di bioprospecting marino e utilizza biologia sintetica e intelligenza artificiale. Il candidato selezionato svilupperà metodi per l'espressione di cluster genici biosintetici, combinando biologia molecolare e ingegneria microbica. È richiesta una laurea di dottorato in biologia sintetica, biotecnologie o discipline affini, con esperienza documentata in ingegneria genetica e analisi di dati biologici. Il ricercatore collaborerà con un dottorando e partner nazionali e internazionali, contribuendo a pubblicazioni scientifiche e presentazioni a conferenze. This is NTNU NTNU is a broad-based university with a technical-scientific profile and a focus in professional education. The university is located in three cities with headquarters in Trondheim. At NTNU, 9,000 employees and 43,000 students work to create knowledge for a better world. You can find more information about working at NTNU and the application process here. About the Job We have a vacancy for a full-time three year researcher at the Department of Biotechnology and Food Science, NTNU. The position is linked to AIMARIA – Accelerating Innovation in Marine AI-Powered Bioprospecting, a Horizon Europe Innovation Action coordinated by NTNU. AIMARIA combines omics, bioinformatics, artificial intelligence, synthetic biology, bioprocess development and sustainability assessment to accelerate the discovery and sustainable production of marine and aquatic natural products. At NTNU, a central part of the project is the development of more predictable and efficient methods for the refactoring and heterologous expression of biosynthetic gene clusters (BGCs). The researcher will have a leading role in developing NTNUs experimental platform for BGC expression. The work will combine molecular biology, microbial strain engineering and synthetic biology with AI-supported design of regulatory DNA elements and expression constructs. The researcher will work closely with a PhD candidate recruited to the same scientific activity and with national and international partners in the AIMARIA consortium. The position is well suited for a researcher who would like to develop and apply advanced synthetic-biology methods to challenging microbial expression systems and contribute to the production of biologically active natural products and enzymes. Your immediate leader is Head of the department. Duties of the position Develop and implement strategies for the design, refactoring and heterologous expression of biosynthetic gene clusters. Design and construct expression systems, including promoters, 5′ untranslated regions and other regulatory DNA elements. Engineer and evaluate microbial host strains for the expression of complex biosynthetic pathways. Analyse and interpret molecular, expression and production data. Coordinate relevant NTNU activities with partners in the AIMARIA consortium. Contribute to project deliverables, scientific reports, presentations and peer-reviewed publications. Present project results at consortium meetings, scientific conferences and relevant stakeholder events. The distribution of duties may be adjusted according to the candidate’s qualifications and the scientific development of the project. Required selection criteria A completed PhD in synthetic biology, molecular biology, biotechnology, microbiology, biochemistry, biochemical engineering or another relevant discipline. Documented hands-on experience in BGC refactoring, molecular cloning, DNA assembly and genetic engineering of microorganisms. Experience with heterologous gene expression, metabolic engineering, pathway engineering or related microbial biotechnology. Ability to plan, conduct, document and analyse experimental research independently. Experience with the analysis and interpretation of biological data. A scientific publication record relevant to the position. Excellent written and oral English language skills. The PhD degree must have been completed and documented by the application deadline. Preferred selection criteria Experience with biosynthetic gene clusters or microbial natural-product pathways. Experience with Streptomyces, actinomycetes or other microbial hosts used for heterologous BGC expression. Experience with assembly, manipulation or expression of large DNA constructs. Experience with promoter engineering, regulatory-sequence design or optimisation of microbial gene expression. Experience with CRISPR-based genome engineering or other advanced strain-engineering methods. Knowledge of bioinformatics, DNA-sequence analysis or computational Annuncio in inglese. Fonte: Euraxess (Commissione europea).